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In this tutorial, I will show you how to configure components of a vacuum filtration station in Bravo Diagnostics.

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In addition, I will explain how to use the Assemble, Disassemble, and Move to Filter tasks for vacuum filtration.

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This video assumes that you already know how to create a device file in the VWorks software and have basic protocol-writing skills.

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If you do not have this background, please view the Device File video first before continuing.

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You can also learn about device files and writing basic protocols in the VWorks Automation Control User Guide.

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To accommodate different assay types, the Bravo Platform supports the vacuum filtration station configurations shown in this diagram.

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In configuration A, the assembly consists of the components in the order shown: the base, the collection plate (because the filtrate will be kept), the filter plate, and the collar.

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In configuration B, the assembly consists of the components in the order shown: the base, the collection plate (because the filtrate will be kept), the collar, and the filter plate.

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In configuration C, the assembly consists of the base, the collar, and the filter plate, which sits on top of the collar.

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There is no collection plate, so the filtrate will be discarded.

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On the Bravo Platform, the vacuum filtration station is an accessory that must be configured in Bravo Diagnostics. The station consists of the base and the collar.

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If the collar sits directly on top of the base, as in configuration C, the filtration station is considered assembled.

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During the protocol, I only have to move the filter plate to the station for filtration.

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If the base and collar have labware between them, as in configurations A and B, the filtration station is considered disassembled.

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During the protocol, I have to move the labware and collar to the base in the desired sequence for the filtration process,

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and remove them from the base after the filtration is finished

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These assembly and disassembly steps can be automated, and I will show how this is done in a minute.

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In my protocol, I’m using configuration B.

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Let’s assume that I have already placed the vacuum filtration base at deck location 1 and the collar at deck location 2.

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Now I’m ready to configure the vacuum filtration station, the base and collar, in Bravo Diagnostics. So, I'll open my device file that already has the Bravo device… 

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and open Bravo Diagnostics.

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I will select the profile I want to use… 

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and go to the Configuration tab.

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I will select deck location 1, …

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and select Accessory.

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In the Bravo Accessories Wizard, …

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first I'll make sure I have the correct deck location.

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Then I will select the accessory I want to configure. In this case, it’s the vacuum filtration station.

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Next, I must set its properties.

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First, I'll select the serial port.

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Next I have to indicate whether the station is assembled or disassembled.

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Remember that I am using configuration B, which has some labware between the base and the collar. So, I'll select Starts disassembled.

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In this case, I’m saying that the base is at location 1. I will have to specify where the collar is on the next page.

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I am using a standard pump, so I'll select that.

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Next, I have to specify the location of the collar.

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Remember that I physically placed the collar at location 2, so I'll select 2.

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I have to specify the type of collar it is. I have a standard collar, so I'll select that.

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And with that, I’m done with the configuration step.

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Here, the software is letting me know that locations 1 and 2 have been changed.

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I do not need to reteach locations 1 and 2, and I do not want to move to a safe Z height right now, so I will click No.

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In the Configuration tab, the graphic is updated.

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I see that the vacuum filtration station base is at location 1 and the collar is at location 2.

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Back in the Profiles tab, I'll verify that locations 1 and 2 have been configured correctly.

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Everything looks good, so I will update this profile to save the changes…

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and then initialize the profile.

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I'll click OK to return to the VWorks window.

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Now I’m ready to add the tasks to assemble the filtration station and perform vacuum filtration in my protocol.

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Let’s say I created the protocol. I’ll open it right now and show you the tasks that I added.

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My protocol is called vacuum filtration protocol, so I will select it.

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Let me expand the protocol area and select the Vacuum Filtration subprocess.

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The graphic shows the deck layout of my Bravo Platform.

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I’ve got a tipbox, a reservoir containing wash buffer, a filter plate, and a collection plate.

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Note that locations 1 and 2 are reserved for the vacuum filtration base and collar, respectively.

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In the Vacuum Filtration subprocess, before the filtration can begin, I have to assemble the filtration station. So I have an Assemble task here.

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In the Assemble task, I selected the assembly order. Remember that the configuration consists of the base, collection plate, and collar.

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So I selected that. Now the software knows the order in which to assemble the components.

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I also selected the collection plate I want to use…

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and its location on the deck, which I left as <auto-select>, because I’ve previously specified its location.

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Notice that the filter plate parameters are grayed out.

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That’s because I selected an assembly order that does not include the filter plate.

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One last parameter I want to point out… The Vacuum Filtration base parameter shows that I have the base at location 1, which is correct.

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After the filtration station is assembled, I can press on the tips.

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move some solution into the filter plate, …

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and then move the filter plate to the assembled filtration station.

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I selected the filter plate I want to move, …

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chose <Auto-select> for the location, …

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and selected the vacuum timing mode. I selected Timed, …

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then specified the amount of time the vacuum should stay on, in seconds. In this case, the vacuum will stay on for 60 seconds.

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I want the robot to hold down the filter plate when the vacuum is on, to ensure a vacuum seal. So I selected this option.

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After the filtration process is finished, I can remove the tips…

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And disassemble the filtration station.

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I selected the filter plate that must be removed, …

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where it should go (in this case, return to its original location), …

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and where the filtration station is currently located.

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During the Disassemble task, the robot will first remove the filter plate and return it to the specified location.

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Then, the robot will remove and return the other components to the locations specified in the Assemble Vacuum task.

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And that’s how you automate the assembly and disassembly of the filtration station and perform vacuum filtration.

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For more information about how to configure the vacuum filtration station on the Bravo deck, see the Bravo User Guide.

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For more information about the Assemble, Disassemble, and Move to Filter tasks, see the VWorks Automation Control User Guide.

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