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In this tutorial, I will show you how to use the Mount and Dismount tasks to mount filter plates on collection plates for spin filtration.

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This video assumes that you already know how to create a device file in the VWorks software and have basic protocol-writing skills.

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If you do not have this background, please view the Device File video first before continuing.

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You can also learn about device files and writing basic protocols in the VWorks Automation Control User Guide.

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The Mount task is typically used to place a filter plate on a reservoir that catches the eluent.

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After the centrifuge process, you can use the Dismount task to remove the filter plate from the reservoir.

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To use the Mount and Dismount tasks, I have to first make sure the filter plate and collection plate are defined correctly in the Labware Editor.

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So, let’s open up the Labware Editor.

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I will go to Tools, and click Labware Editor.

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I'll select my filter plate.

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Among other specifications in its definition, I must make sure that it has the Filter plate Base Class, …

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and it Can mount another labware.

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For my collection plate, …

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I have to make sure it has a Microplate Base Class…

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and it can be mounted by another labware, such as my filter plate.

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Now I am ready to add the Mount and Dismount tasks in my protocol.

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Let’s say I created the protocol. I’ll show you how I’ve set up the processes and tasks.

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In my protocol, each labware involved in the Mount and Dismount tasks should be set up as an independent process in the protocol.

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Here I have a process for the filter plate … 

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and a separate process for the collection plate.

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In this protocol, the Filter Plate downstacks from the specified BenchCel stacker…

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and moves to Bravo deck location 8.

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It then goes through some liquid-handling tasks, as shown here in the pipette step subprocess.

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At the end of the liquid-handling routine, the Collection Plate process is spawned.

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The Filter Plate has to wait until the Collection Plate is ready.

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Notice that when I added the Wait For task, I named it Wait for Filter.

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The name helps me identify and reference the correct labware process later on. I’ll show that in a minute.

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When the Filter Plate is finished, it returns to the specified BenchCel stacker.

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The Collection Plate process is held back until it is spawned. At that point, …

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the Collection Plate downstacks from the specified BenchCel stacker…

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and moves to Bravo deck location 9.

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Now it’s ready to be mounted, but by which labware?

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In the Task Parameters area, notice that I selected Wait for Filter. This is the reference to the Wait For task in the Filter Plate process.

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The eluent is collected during the centrifuge process.

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When finished, the Dismount task removes the Filter Plate from the Collection Plate.

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Notice that the Dismount task does not have any task parameters.

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After the dismount, the Collection Plate returns to the specified BenchCel stacker.

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And that’s how you use the Mount and Dismount tasks. For more information about the Mount and Dismount tasks, see the VWorks Automation Control User Guide.

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