1 0:00:00,00 --> 0:00:03,07 2 0:00:03,07 --> 0:00:11,27 In the previous videos, I created a protocol that includes a hit-picking routine, serial dilution routine, and plate replication routine. 3 0:00:11,27 --> 0:00:20,04 In this video, I’ll show you how to compile the protocol, check for and troubleshoot errors, and simulate protocol runs. 4 0:00:20,04 --> 0:00:24,22 After I finish writing a protocol, I should compile it and check for errors. 5 0:00:24,22 --> 0:00:30,22 I'll first display the Main Log so that I can see the events and errors during the compile process. 6 0:00:30,22 --> 0:00:44,03 Then I'll click Compile… OK, I see that I have some warning messages, all having to do with the replication plates at Bravo locations 1 and 4. 7 0:00:44,03 --> 0:00:54,07 I can disregard these, because I know these locations are both in the corner and on the edge of the Bravo deck. So they will not interfere with any liquid-handling tasks. 8 0:00:54,07 --> 0:01:05,02 The compile process did not find any errors, so the protocol is in good shape. Before I do anything else, I better save this protocol file. 9 0:01:05,02 --> 0:01:11,20 Next, I want to simulate the run and see if there are any issues that might cause problems, like a deadlock. 10 0:01:11,20 --> 0:01:17,01 Before I start the simulation, I like to optimize the protocol display area so that I can monitor the progress visually. 11 0:01:17,01 --> 0:01:23,18 So I'll click the pushpin button here to hide the Task Parameters area. 12 0:01:23,18 --> 0:01:28,23 And I'll hide the Available Tasks area similarly. 13 0:01:28,23 --> 0:01:37,07 Now I’ll scroll to the top so that the Destination plate Process, which contains everything, is visible. All the way to the left here… 14 0:01:37,07 --> 0:01:43,17 I still can’t see the entire Destination process, so let me zoom out a little. I'll click in the protocol. 15 0:01:43,17 --> 0:01:52,26 While holding down the Control key, I'll rotate the mouse wheel. Now I can see everything. 16 0:01:52,26 --> 0:01:57,23 So I'll go ahead and start the simulation. 17 0:01:57,23 --> 0:02:06,02 In the Run Configuration Wizard, I can specify the number of times I want to run this protocol. I'll leave it at one time for now. 18 0:02:06,02 --> 0:02:09,28 In the box below, I can verify that the information is correct. 19 0:02:09,28 --> 0:02:16,26 On the next page, I can select when I want to run this protocol. I want to run it now, so I'll leave the default selection. 20 0:02:16,26 --> 0:02:24,11 On the next page, I have to specify the starting states of the tipboxes I am tracking, which are the tips for hit-picking. 21 0:02:24,11 --> 0:02:33,03 For the Tips On box, I want to specify that the box is full. And here it shows all 384 tips are selected, so that’s ready to go. 22 0:02:33,03 --> 0:02:38,28 And for the Tips Off box, I want to start with an empty box. And it says here it’s empty. 23 0:02:38,28 --> 0:02:43,17 I don’t have any barcodes… And I do not wish to add any notes at this time. 24 0:02:43,17 --> 0:02:46,29 The simulation starts as soon as I click Finish. 25 0:02:46,29 --> 0:02:56,03 Every event happening during the simulation is shown in the Main Log area. But it’s scrolling by so fast! I can disable the automatic scrolling. 26 0:02:56,03 --> 0:03:06,27 To do this, I can right-click the Main Log area and select Stop Scrolling. Or I can display the Progress tab and monitor the run from there. 27 0:03:06,27 --> 0:03:14,24 But right now, I’d like to monitor what’s going on up here in the Main Protocol area, where I can get an overall view of the entire run. 28 0:03:14,24 --> 0:03:22,21 The green dot shows which task is running… Everything appears to be running smoothly so far… 29 0:03:22,21 --> 0:03:32,00 A message appears when the simulation is finished. There weren’t any deadlock or other errors during simulation so the protocol is ready to be tested in a dry run. 30 0:03:32,00 --> 0:03:36,02 After it passes that run, I can run it using real plates. 31 0:03:36,02 --> 0:03:39,09