1 00:00:07,000 --> 00:00:12,312 Evaporative light scattering is an ideal detection technique for LC-separated analytes 2 00:00:12,347 --> 00:00:14,790 that do not have light-absorbing chromophores. 3 00:00:14,825 --> 00:00:20,431 And because this technique does not rely on optical properties, tedious sample preparation 4 00:00:20,466 --> 00:00:23,174 and derivatization can be eliminated. 5 00:00:23,634 --> 00:00:31,530 The new Agilent 1290 Infinity ELSD takes this capability further by facilitating sub-ambient operation 6 00:00:31,630 --> 00:00:35,032 for unrivalled detection of semi-volatile compounds. 7 00:00:36,300 --> 00:00:41,892 The three key stages of nebulization, eluent evaporation and optical detection, 8 00:00:41,927 --> 00:00:47,600 are optimized by design to achieve maximum sensitivity and highest reproducibility 9 00:00:47,650 --> 00:00:50,131 for accurate and reliable results. 10 00:00:50,550 --> 00:00:56,260 The nebulizer uses a stream of inert gas to transform the eluent from the LC column 11 00:00:56,295 --> 00:00:59,466 into a fine vapor of uniformly sized droplets. 12 00:00:59,501 --> 00:01:06,051 Control of nebulizer temperature and gas flow provide for excellent stability and reproducibility. 13 00:01:06,586 --> 00:01:13,650 A study of 50 replicate caffeine injections showed peak height variations of less than 2.0 % 14 00:01:13,685 --> 00:01:17,305 - thanks to the design of the high-precision glass nebulizer. 15 00:01:17,750 --> 00:01:20,958 The evaporator tube temperature is precisely controlled 16 00:01:21,100 --> 00:01:24,888 and removes the solvent from the less-volatile analyte particles. 17 00:01:25,523 --> 00:01:29,693 The volume of the tube is extremely low, minimizing peak dispersion. 18 00:01:30,300 --> 00:01:34,550 In the optical chamber, a short-wavelength, high-power blue laser 19 00:01:34,650 --> 00:01:38,319 maximizes the scattering from the small analyte particles. 20 00:01:38,354 --> 00:01:43,816 Advanced detection electronics and digital signal processing minimize baseline noise 21 00:01:43,951 --> 00:01:46,399 to ensure highest sensitivity. 22 00:01:47,000 --> 00:01:50,818 The 1290 Infinity ELSD deploys patented technology 23 00:01:50,853 --> 00:01:54,500 to remove the solvent at temperatures as low as 10 degrees centigrade, 24 00:01:54,600 --> 00:01:58,375 ensuring compounds are not evaporated prior to detection. 25 00:01:58,402 --> 00:02:03,000 This provides for sensitive, reliable quantification of semi-volatile compounds 26 00:02:03,100 --> 00:02:08,908 that simply cannot be detected with other evaporative light scattering or aerosol-based detectors. 27 00:02:09,700 --> 00:02:15,320 The 1290 Infinity ELSD is unique in its ability to run gas flow gradients, 28 00:02:15,400 --> 00:02:20,435 which adjust the size of the nebulized droplets, compensating for mobile phase changes 29 00:02:20,510 --> 00:02:23,490 and thereby achieving uniform detector response. 30 00:02:24,000 --> 00:02:28,900 The detector operates over the widest flow range and with the steepest gradients 31 00:02:29,000 --> 00:02:32,933 without the need to split the flow or to exchange nebulizers. 32 00:02:32,968 --> 00:02:39,869 The excellent baseline stability and low dispersion characteristics make it ideal for UHPLC applications. 33 00:02:40,400 --> 00:02:43,868 By careful control of the gas flow during evaporation, 34 00:02:43,903 --> 00:02:48,493 solvents such as dimethyl sulfoxide can be removed completely, 35 00:02:48,528 --> 00:02:54,387 facilitating accurate compound screening without the need for extensive sample preparation. 36 00:02:55,800 --> 00:03:02,372 Evaporative light scattering is also an ideal detection technique for supercritical fluid chromatography. 37 00:03:02,650 --> 00:03:06,700 With thermally insulated bulkhead fittings and a heated nebulizer, 38 00:03:06,800 --> 00:03:12,849 the 1290 Infinity ELSD can easily be coupled to an SFC system without modification. 39 00:03:12,884 --> 00:03:18,738 And because SFC provides faster, higher resolution separations than conventional HPLC, 40 00:03:18,773 --> 00:03:22,384 laboratory throughput can be increased significantly. 41 00:03:23,300 --> 00:03:29,050 The Agilent 1290 Infinity ELSD offers a wealth of productivity benefits 42 00:03:29,120 --> 00:03:34,953 such as low temperature evaporation, excellent reproducibility, uniform detector response, 43 00:03:34,988 --> 00:03:42,468 widest flow range, lowest dispersion, DMSO transparency and SFC compatibility. 44 00:03:44,400 --> 00:03:50,200 The Agilent 1290 Infinity ELSD is the detector of choice for: 45 00:03:50,250 --> 00:04:05,991 Analytical and 2-dimensional LC, LC/MS, SFC, Preparative LC, and GPC/SEC 46 00:04:07,250 --> 00:04:14,525 The Agilent 1290 Infinity ELSD DETECTS VIRTUALLY EVERYTHING YOUR LC SEPARATES. 47 00:04:17,763 --> 00:04:22,930 True universal detection is just one of a wave of innovative technologies 48 00:04:22,965 --> 00:04:29,954 to ensure your Agilent 1200 Infinity Series LC stays at the forefront of separation potential.