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Agilent Buffer Advisor software eliminates the tedious 
and error-prone steps of buffer preparation, 

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buffer blending and pH scouting, which are 
traditionally performed manually during 

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method development for 
ion exchange chromatography. 

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Combined with the 
Agilent 1260 Infinity Bio-inert LC system, 

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Buffer Advisor software facilitates dynamic mixing 
of solvents to create salt or pH gradients 

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from only four stock solutions. 

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This significantly reduces the time 
required for buffer preparation.

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Four stock solutions are all you need:

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Acid

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Base

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Water

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Salt solution

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From these four stock solutions, buffers at different 
pH values and concentrations can be created easily. 

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Buffer Advisor software helps you to create 
a salt gradient by generating a timetable 

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for the 1260 Infinity Bio-inert LC pump. 

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An increasing amount of salt solution is mixed 
with the acidic and basic buffer components, 

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and with water for dilution.

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You can also use Buffer Advisor software to generate 
pH gradients by combining buffers of different pH. 

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First, a low pH buffer binds 
the proteins to the column. 

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Then a high pH buffer elutes 
the proteins from the column.

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Through a single control panel in 
Buffer Advisor software, 

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you can enter all relevant parameters 
to create an optimized gradient timetable.

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Select buffer and gradient type 

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Enter gradient timetable

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Review composition of stock solutions and adjust.

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In the gradient display section 
you can process the gradient 

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and review the optimized gradient 
with compensation steps.

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A wide range of buffers are available in the software.

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Simply select buffers from a dropdown menu according 
to the pH range required by your application. 

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Also selectable is the gradient type 
as well as the mode of ion exchange.

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The gradient table in Buffer Advisor software 
allows you to create a salt gradient by entering pH, 

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salt concentration and buffer concentration.

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You can also review and print out recipes 
for your chosen stock solutions.

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Adding salt ions to a buffer influences its pH.

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 To compensate for this effect 
and to maintain a stable pH, 

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Buffer Advisor software
 calculates additional mixing steps.

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The optimized gradient timetable 
can now be saved as an XML-format file, 

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which can be later imported into 
Agilent OpenLAB CDS. 

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This file sets the solvent blending for the 
1260 Infinity Bio-inert Quaternary Pump 

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in the module's timetable that in turn becomes 
part of the software's method.

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In this example of pH scouting with marker proteins, 
three salt gradient tables for different pH values 

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were created with Buffer Advisor software. 

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Simplify method development of protein separations 
with Agilent Buffer Advisor software 

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and eliminate the challenges of traditional 
preparation of buffers for pH and salt gradients.

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Fast Buffer Scouting is just one of a wave 
of innovative Agilent technologies 

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for ensuring your Agilent 1200 Infinity Series LC 
stays at the forefront of separation potential.